Recombinant Anti-EPS15R antibody [EP1146Y] (ab76004)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1146Y] to EPS15R
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EPS15R antibody [EP1146Y]
See all EPS15R primary antibodies -
Description
Rabbit monoclonal [EP1146Y] to EPS15R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EPS15R aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UBC2 -
Positive control
- HeLa, 293T and SHSY5Y cell lysates; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1146Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/20000. Predicted molecular weight: 94 kDa.
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Notes |
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WB
1/10000 - 1/20000. Predicted molecular weight: 94 kDa. |
Target
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Function
Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 3 EH domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by EGFR. -
Cellular localization
Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits. - Information by UniProt
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Database links
- Entrez Gene: 58513 Human
- Entrez Gene: 13859 Mouse
- Entrez Gene: 313474 Rat
- Omim: 600051 Human
- SwissProt: Q9UBC2 Human
- SwissProt: Q60902 Mouse
- Unigene: 654639 Human
- Unigene: 744842 Human
see all -
Alternative names
- EP15R_HUMAN antibody
- epidermal growth factor receptor pathway substrate 15- ike 1 antibody
- Epidermal growth factor receptor substrate 15 like 1 antibody
see all
Images
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All lanes : Anti-EPS15R antibody [EP1146Y] (ab76004) at 1/20000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : SHSY5Y cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 108,125,76 kDa why is the actual band size different from the predicted?
There are three isoforms with MW at 125, 108 and 76 kDa respectively.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab76004 has been referenced in 3 publications.
- Poświata A et al. Endocytic trafficking of GAS6-AXL complexes is associated with sustained AKT activation. Cell Mol Life Sci 79:316 (2022). PubMed: 35622156
- Cendrowski J et al. Splicing variation of BMP2K balances abundance of COPII assemblies and autophagic degradation in erythroid cells. Elife 9:N/A (2020). PubMed: 32795391
- Fukushima T et al. Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity. Nat Commun 6:6780 (2015). PubMed: 25879670