Overview

  • Product nameAnti-EPS15R antibody [EP1146Y]
    See all EPS15R primary antibodies
  • Description
    Rabbit monoclonal [EP1146Y] to EPS15R
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EPS15R aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBC2

  • Positive control
    • HeLa, 293T and SHSY5Y cell lysates; A431 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab76004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Predicted molecular weight: 94 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionSeems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
    • Sequence similaritiesContains 1 EF-hand domain.
      Contains 3 EH domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues by EGFR.
    • Cellular localizationCell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
    • Information by UniProt
    • Database links
    • Alternative names
      • EP15R_HUMAN antibody
      • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
      • Epidermal growth factor receptor substrate 15 like 1 antibody
      • Epidermal growth factor receptor substrate 15-like 1 antibody
      • epidermal growth factor receptor substrate EPS15R antibody
      • Eps15 related protein antibody
      • Eps15-related protein antibody
      • EPS15L1 antibody
      • Eps15R antibody
      see all

    Anti-EPS15R antibody [EP1146Y] images

    • ab76004 staining EPS15R in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

    • ab76004 at 1/100-1/250 dilution staining EPS15R in A431 cells by Immunofluorescence.
    • All lanes : Anti-EPS15R antibody [EP1146Y] (ab76004) at 1/20000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : SHSY5Y cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 94 kDa
      Observed band size : 76,108,125 kDa (why is the actual band size different from the predicted?)
      There are three isoforms with MW at 125, 108 and 76 kDa respectively.

    References for Anti-EPS15R antibody [EP1146Y] (ab76004)

    ab76004 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76004.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"