Overview

  • Product name
    Anti-EPS15R antibody [EP1147Y]
    See all EPS15R primary antibodies
  • Description
    Rabbit monoclonal [EP1147Y] to EPS15R
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human EPS15R was used as an immunogen

  • Positive control
    • HeLa cell membrane or nuclear cell lysate Human breast carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab53006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
IP 1/70.
ICC 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
  • Sequence similarities
    Contains 1 EF-hand domain.
    Contains 3 EH domains.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by EGFR.
  • Cellular localization
    Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
  • Information by UniProt
  • Database links
  • Alternative names
    • EP15R_HUMAN antibody
    • epidermal growth factor receptor pathway substrate 15- ike 1 antibody
    • Epidermal growth factor receptor substrate 15 like 1 antibody
    • Epidermal growth factor receptor substrate 15-like 1 antibody
    • epidermal growth factor receptor substrate EPS15R antibody
    • Eps15 related protein antibody
    • Eps15-related protein antibody
    • EPS15L1 antibody
    • Eps15R antibody
    see all

Images

  • Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size : 94 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab53006, at a 1/50 dilution, staining Human EPS15R in Breast Carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.

References

This product has been referenced in:
  • Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). Read more (PubMed: 19704019) »
  • Chen C & Zhuang X Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A 105:11790-5 (2008). Read more (PubMed: 18689690) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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