Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1µg/ml.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tested in Human Breast Carcinoma showing staining of cancer cells infiltrated in a breast lobe.

Target

  • Function
    Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificity
    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similarities
    Belongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

Images

  • ab38329 (5µg/ml) staining paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab38329 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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