The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1µg/ml.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tested in Human Breast Carcinoma showing staining of cancer cells infiltrated in a breast lobe.
FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
Sequence similaritiesBelongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with SHB.
Post-translational modificationsPhosphorylated by several receptor tyrosine kinases.