Overview

  • Product nameAnti-EPS8 antibody
    See all EPS8 primary antibodies
  • Description
    Mouse polyclonal to EPS8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human EPS8 (NP_004438.3, amino acids 1-822)

  • Positive control
    • Human liver tissue lysate, K562 cell lysate, EPS8 transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 92 kDa.

Target

  • FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similaritiesBelongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

Anti-EPS8 antibody images

  • Anti-EPS8 antibody (ab89477) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
  • Anti-EPS8 antibody (ab89477) at 1/500 dilution + K-562 cell lysate at 50 µg

    Predicted band size : 92 kDa
  • All lanes : Anti-EPS8 antibody (ab89477) at 1/500 dilution

    Lane 1 : EPS8 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 92 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-EPS8 antibody (ab89477)

ab89477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"