Overview

  • Product nameAnti-EPS8 antibody
    See all EPS8 primary antibodies
  • Description
    Rabbit polyclonal to EPS8
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 761- 822 (DELRTVCPEG ARVYSQITVQ KAALEDSSGS SELQEIMRRR QEKISAAASD SGVESFDEGS SH) of Human EPS8 (NP_004438).

  • Positive control
    • WB: H1299, HeLa cell lysates IHC-P: Gastric CA

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by antigen affinity chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/250.

Target

  • FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similaritiesBelongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

Anti-EPS8 antibody images

  • All lanes : Anti-EPS8 antibody (ab96144) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 92 kDa
  • ab96144, at 1/100 dilution, staining EPS8 in paraffin embedded Gastric CA by Immunohistochemical analysis.

References for Anti-EPS8 antibody (ab96144)

ab96144 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"