Overview

  • Product name
  • Description
    Rabbit polyclonal to EPS8
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 761- 822 (DELRTVCPEG ARVYSQITVQ KAALEDSSGS SELQEIMRRR QEKISAAASD SGVESFDEGS SH) of Human EPS8 (NP_004438).

  • Positive control
    • WB: H1299, HeLa cell lysates IHC-P: Gastric CA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by antigen affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function
    Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
  • Tissue specificity
    Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
  • Sequence similarities
    Belongs to the EPS8 family.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with SHB.
  • Post-translational
    modifications
    Phosphorylated by several receptor tyrosine kinases.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epidermal growth factor receptor kinase substrate 8 antibody
    • Epidermal growth factor receptor pathway substrate 8 antibody
    • EPS 8 antibody
    • EPS8 antibody
    • EPS8_HUMAN antibody
    see all

Anti-EPS8 antibody images

  • All lanes : Anti-EPS8 antibody (ab96144) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 92 kDa
  • ab96144, at 1/100 dilution, staining EPS8 in paraffin embedded Gastric CA by Immunohistochemical analysis.

References for Anti-EPS8 antibody (ab96144)

This product has been referenced in:
  • Mok KW  et al. Rictor/mTORC2 regulates blood-testis barrier dynamics via its effects on gap junction communications and actin filament network. FASEB J 27:1137-52 (2013). Read more (PubMed: 23288930) »
  • Qian X  et al. Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis. Am J Physiol Endocrinol Metab 304:E757-69 (2013). IF . Read more (PubMed: 23403943) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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