The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
1/100 - 1/250. antigen retrieval is recommended.
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
Sequence similaritiesBelongs to the EPS8 family. Contains 1 PH domain. Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with SHB.
Post-translational modificationsPhosphorylated by several receptor tyrosine kinases.