Overview

  • Product nameAnti-EPS8 antibody [EPR6112]
    See all EPS8 primary antibodies
  • Description
    Rabbit monoclonal [EPR6112] to EPS8
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of Human EPS8.

  • Positive control
    • HeLa, A375, 293T, and K562 cell lysates, Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/100 - 1/250. antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionUpon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets.
    • Tissue specificityExpressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
    • Sequence similaritiesBelongs to the EPS8 family.
      Contains 1 PH domain.
      Contains 1 SH3 domain.
    • DomainThe SH3 domain mediates interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated by several receptor tyrosine kinases.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epidermal growth factor receptor kinase substrate 8 antibody
      • Epidermal growth factor receptor pathway substrate 8 antibody
      • EPS 8 antibody
      • EPS8 antibody
      • EPS8_HUMAN antibody
      see all

    Anti-EPS8 antibody [EPR6112] images

    • All lanes : Anti-EPS8 antibody [EPR6112] (ab124882) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 92 kDa
    • Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.

    References for Anti-EPS8 antibody [EPR6112] (ab124882)

    ab124882 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124882.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"