Overview

  • Product name
    Anti-EPS8L1 antibody
  • Description
    Rabbit polyclonal to EPS8L1
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog
  • Immunogen

    A region within synthetic peptide: ERRAAALRAT QEELQRDRSP AAETPPLQRR PSVRAVISTV ERGAGRGRPQ, corresponding to N terminal amino acids 37-86 of Human EPS8L1

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human placental tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab58687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
  • Tissue specificity
    Detected in placenta.
  • Sequence similarities
    Belongs to the EPS8 family.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DRC3 antibody
    • Epidermal growth factor receptor kinase substrate 8 like protein 1 antibody
    • Epidermal growth factor receptor kinase substrate 8-like protein 1 antibody
    • Epidermal growth factor receptor pathway substrate 8 related protein 1 antibody
    • Epidermal growth factor receptor pathway substrate 8-related protein 1 antibody
    • EPS8 like 1 antibody
    • EPS8 like protein 1 antibody
    • EPS8-like protein 1 antibody
    • EPS8-related protein 1 antibody
    • EPS8L 1 antibody
    • Eps8l1 antibody
    • EPS8R1 antibody
    • ES8L1 antibody
    • ES8L1_HUMAN antibody
    • FLJ20258 antibody
    • MGC23164 antibody
    • MGC4642 antibody
    • PP10566 antibody
    see all

Images

  • Anti-EPS8L1 antibody (ab58687) at 1.25 µg/ml + Hep2 cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution.

    Predicted band size : 80 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human placenta tissue labelling EPS8L1 with ab58687 at 4-8µg/ml. Arrows indicate positively labelled decidual cells. Magnification: 400X.

References

ab58687 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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