The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20000.
IHC-P: 1/50 - 1/100. For antigen retrieval, boil in a pressure cooker for 2-3 min. Use the polymer system for signal enhancing.
WB: 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionStimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
Tissue specificityDetected in placenta.
Sequence similaritiesBelongs to the EPS8 family. Contains 1 SH3 domain.