Anti-epsilon Tubulin antibody [TUB-11] (ab11322)

Overview

  • Product nameAnti-epsilon Tubulin antibody [TUB-11]
    See all epsilon Tubulin primary antibodies
  • Description
    Mouse monoclonal [TUB-11] to epsilon Tubulin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 352-366 of Human epsilon Tubulin.

  • Positive control
    • whole extract of cultured chicken fibroblasts.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberTUB-11
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52.9 kDa).

Target

References for Anti-epsilon Tubulin antibody [TUB-11] (ab11322)

ab11322 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem)
Specification embryonic stem
Fixative Methanol
Permeabilization Yes - 0.1% triton-x-100 in PBS
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Prof. Reiter Lab

Verified customer

Submitted Apr 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"