Overview

  • Product nameAnti-Epsin 2 antibody
  • Description
    Rabbit polyclonal to Epsin 2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Epsin 2.

  • Positive control
    • Extracts from HepG2 cells or HUVEC cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab74942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
ELISA 1/20000.

Target

  • FunctionPlays a role in the formation of clathrin-coated invaginations and endocytosis.
  • Tissue specificityHighest expression is found in brain. Detected at lower levels in lung and liver.
  • Sequence similaritiesBelongs to the epsin family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • DomainThe NPF repeat domain is involved in EPS15 binding.
    The DPW repeat domain is involved in AP-2 and clathrin binding.
  • Post-translational
    modifications
    Ubiquitinated.
  • Cellular localizationCytoplasm. Cytoplasmic vesicle > clathrin-coated vesicle. In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • EHB21 antibody
    • EPN 2 antibody
    • Epn2 antibody
    • EPN2_HUMAN antibody
    • EPS 15 interacting protein 2 antibody
    • EPS-15-interacting protein 2 antibody
    • Eps15 binding protein antibody
    • Epsin 2 antibody
    • Epsin-2 antibody
    • Epsin2 antibody
    • KIAA1065 antibody
    • OTTHUMP00000065808 antibody
    • OTTHUMP00000065809 antibody
    • OTTHUMP00000065810 antibody
    • OTTHUMP00000065811 antibody
    • OTTHUMP00000065889 antibody
    see all

Anti-Epsin 2 antibody images

  • All lanes : Anti-Epsin 2 antibody (ab74942) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HUVEC cells
    Lane 3 : Extracts from HepG2 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Epsin 2 antibody (ab74942)

ab74942 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"