Overview

  • Product nameAnti-EPX antibody
    See all EPX primary antibodies
  • Description
    Rabbit polyclonal to EPX
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human EPX.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Thymus; Spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab65319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).

Target

  • FunctionMediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.
  • Involvement in diseaseDefects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
  • Cellular localizationCytoplasmic granule. Cytoplasmic granules of eosinophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Eosinophil peroxidase [Precursor] antibody
    • Eosinophil peroxidase antibody
    • Eosinophil peroxidase heavy chain antibody
    • EPER antibody
    • EPO antibody
    • EPP antibody
    • EPX antibody
    • EPX PEN antibody
    • PERE antibody
    • PERE_HUMAN antibody
    see all

Anti-EPX antibody images

  • All lanes : Anti-EPX antibody (ab65319) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,55 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-EPX antibody (ab65319)

ab65319 has not yet been referenced specifically in any publications.

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