Overview

  • Product nameAnti-ERAP2 antibody
    See all ERAP2 primary antibodies
  • Description
    Mouse polyclonal to ERAP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human ERAP2 protein (AAH17927, 350 a.a)

  • Positive control
    • Human kidney tissue lysate. ERAP2 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 110 and 40 kDa (predicted molecular weight: 110 kDa; Isoform 4, 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • Cellular localizationEndoplasmic reticulum membrane; Single-pass type II membrane protein.
    • Database links
    • Alternative names
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Anti-ERAP2 antibody images

    • Anti-ERAP2 antibody (ab69037) at 1/500 dilution + Human kidney lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
    • All lanes : Anti-ERAP2 antibody (ab69037) at 1/500 dilution

      Lane 1 : ERAP2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 110 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References for Anti-ERAP2 antibody (ab69037)

    This product has been referenced in:
    • Kuiper JJ  et al. A genome-wide association study identifies a functional ERAP2 haplotype associated with birdshot chorioretinopathy. Hum Mol Genet 23:6081-7 (2014). Human . Read more (PubMed: 24957906) »
    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab69037.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"