Overview

  • Product nameAnti-ERAP2 antibody
    See all ERAP2 primary antibodies
  • Description
    Goat polyclonal to ERAP2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Peptide with sequence C-

    QYLKKFSYRNAKND

    , corresponding to internal sequence amino acids 480-493 of human ERAP2 (NP_071745.1).

  • Positive control
    • Human spleen lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • Cellular localizationEndoplasmic reticulum membrane; Single-pass type II membrane protein.
    • Database links
    • Alternative names
      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Anti-ERAP2 antibody images

    • Anti-ERAP2 antibody (ab82142) at 0.5 µg/ml + human spleen lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 hour

    References for Anti-ERAP2 antibody (ab82142)

    ab82142 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab82142.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"