The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
Endoplasmic reticulum membrane; Single-pass type II membrane protein.