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TAENPEYLGLDVPV, corresponding to C terminal amino acids 1242 - 1255 of Human c-ErbB2.
Our Abpromise guarantee covers the use of ab16901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 2.5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa).|
|IP||Use 1µg for 106 cells.|
|Flow Cyt||1/50 - 1/100.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling ErbB 2 with ab16901 at 1/400 dilution. the tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a acetate buffer pH6. An undiluted goat anti-mouse HRP conjugated seconday antibody was used.
This image is courtesy of an anonymous abreview.
Blocking: 5% milk for 1 hour at 25°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Mamma tumor Her2Neu tissue labeling ErbB 2 with ab16901.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labeling ErbB 2 with ab16901.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"