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Our Abpromise guarantee covers the use of ab2428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||1/200. Can be blocked with ErbB 2 peptide (ab2429).|
|ICC||Use at an assay dependent concentration.|
ab2428 staining ErbB 2 in human breast cancer, tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking in 10% serum for 1 hour at 20°C.The primary antibody was diluted, 1/100 in TBS and incubated with sample for 12 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary. Left hand-side image show staining with ab2428 and developed using DAB detection system while right hand-side shows Isotype control staining with anti-rabbit IgG and DAB.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"