Overview

  • Product nameAnti-ErbB 2 antibodySee all ErbB 2 primary antibodies ...
  • Description
    Rabbit polyclonal to ErbB 2
  • Tested applicationsIHC-P, IHC-Fr, IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • SK-BR-3 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/200. Can be blocked with ErbB 2 peptide (ab2429).
ICC Use at an assay dependent concentration.

Target

  • FunctionProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • Tissue specificityExpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • Involvement in diseaseHereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
  • Cellular localizationCytoplasm. Nucleus and Cell membrane. Cytoplasm > perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • c erb B2 antibody
    • C erb B2/neu protein antibody
    • C erbB 2 antibody
    • C-erbB2 antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • Erb B2 antibody
    • erbb2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/neu antibody
    • HER2 antibody
    • Her2/neu antibody
    • Herstatin antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU Proto Oncogene antibody
    • Neuro Glioblastoma Derived Oncogene Homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185 ErbB2 antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor Protein Tyrosine Kinase ErbB2 Precursor antibody
    • Receptor tyrosine protein kinase erbB 2 antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

Anti-ErbB 2 antibody images

  • ab2428 at a 1:200 dilution staining SK-BR-3 whole cell lysate.
  • Western blot of whole cell lysates of cultured cells, used at a concentration of 1:1000, incubated overnight at 4C, and visualized with an HRP conjugated secondary, with a chemilumenescent detection method. Review by Todd Gocken submitted 20 July 2004
  • ab2428 staining ErbB 2 in human breast cancer, tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking in 10% serum for 1 hour at 20°C.The primary antibody was diluted, 1/100 in TBS and incubated with sample for 12 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary. Left hand-side image show staining with ab2428 and developed using DAB detection system while right hand-side shows Isotype control staining with anti-rabbit IgG and DAB.

    See Abreview

References for Anti-ErbB 2 antibody (ab2428)

This product has been referenced in:
  • Wakefield A  et al. Bcl3 selectively promotes metastasis of ERBB2-driven mammary tumors. Cancer Res 73:745-55 (2013). WB ; Mouse . Read more (PubMed: 23149915) »
  • Huang Z  et al. Stretch-induced fetal type II cell differentiation is mediated via ErbB1-ErbB4 interactions. J Biol Chem 287:18091-102 (2012). IP . Read more (PubMed: 22493501) »

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (heart)
Specification heart
Permeabilization Yes - saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 25 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for your enquiry.

I can confirm that ErbB2 is known to be expressed in breast issue and highly expressed in many breast cancers. Breast tumor tissue would therefore be a positive control to consider. You may want to do consider som...

Read More

Thank you for contacting us.

Products;

PAX6; http://www.abcam.com/PAX6-antibody-Stem-Cell-Marker-ab5790.html

NeuN; http://www.abcam.com/FOX3NeuN-antibody-ab104225.html

Protcol;

http://www.abcam.com/index.htm...

Read More

Thank you for contacting us. Your credit note ID is xxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

Read More

Thank you for your response.

The protocol seems to be fine and the antibody should work at a dilution of 1/100. At this stage it is difficult to identify the source of the problem, it could be the product has been damaged during storage or sh...

Read More

Thank you for your response and for confirmation.

It was important to know if the detection system was working fine (ab64264). This means that the problem comes from the primary antibody (ab2428).

I can offer you and your troubled c...

Read More

Thank you for your response.

As I understand ab64264: Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit which was used as detection worked fine.

The customer is having problem with only the primary antibody ab2428.

Read More

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

Read More

Thank you for your enquiry.


I can confirm that ErbB2 and HER 2 are alternative names for the same protein:

http://en.wikipedia.org/wiki/HER2/neu

I hope this will be helpful to you. If you have any further questions...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"