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Synthetic peptide corresponding to a site on the internal domain of the C-erbB-2 oncoprotein.
C-erbB-2 oncogene codes for a membrane protein, Neu protein. In several studies the amplification of C-erbB-2 oncogene in carcinomas has been reported. Overexpression of the Neu oncogene protein can be demonstrated by this antibody.
Our Abpromise guarantee covers the use of ab8054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. Fix with acetone, 10 minutes at room temperature.|
|IHC-P||1/5 - 1/10.|
|WB||1/1000. PubMed: 17459787|
Membrane localisation of ErbB2 in the breast cancer cell line SK-Br-3 (green). Nuclei are counterstained with DAPI (blue). This picture was kindly submitted as part of the review by Andy Massey.