Recombinant
RabMAb

Anti-ErbB 2 antibody [EP1045Y] - BSA and Azide free (ab194979)

Overview

  • Product name
    Anti-ErbB 2 antibody [EP1045Y] - BSA and Azide free
    See all ErbB 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1045Y] to ErbB 2 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human ErbB 2 (UniProt P04626).

  • Positive control
    • SKBR-3 cell lysate; Human breast carcinoma tissue; SKBR cells
  • General notes

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).

Please check the parent abID, ab134182, for more information on dilutions.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • Tissue specificity
    Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • Involvement in disease
    Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
  • Cellular localization
    Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • C erb B2/neu protein antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • Erb b2 receptor tyrosine kinase 2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/NEU antibody
    • HER2 antibody
    • Herstatin antibody
    • Human epidermal growth factor receptor 2 antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU proto oncogene antibody
    • Neuro/glioblastoma derived oncogene homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

Images

  • Anti-ErbB 2 antibody [EP1045Y] - BSA and Azide free (ab194979) + SKBR-3 (human mammary gland adenocarcinoma) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 137 kDa
    Observed band size: 185 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

References

This product has been referenced in:
  • Chan KK  et al. Targeting estrogen receptor subtypes (ERa and ERß) with selective ER modulators in ovarian cancer. J Endocrinol 221:325-36 (2014). Human . Read more (PubMed: 24819599) »
  • Sollome JJ  et al. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal 26:70-82 (2013). Human . Read more (PubMed: 24036211) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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