Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] (ab53290)

Overview

  • Product nameAnti-ErbB 2 (phospho Y1139) antibody [EP1046Y]
    See all ErbB 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1046Y] to ErbB 2 (phospho Y1139)
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding tyrosine 1139 of human ErbB 2.

  • Positive control
    • WB: SKBR3 and A431 cell lysates treated with EGF.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab53290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 138 kDa).
IP 1/30.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF,IHC-Fr or IHC-P.
  • Target

    • FunctionProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • Tissue specificityExpressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • Involvement in diseaseHereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • Cellular localizationCytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • C erb B2/neu protein antibody
      • CD340 antibody
      • CD340 antigen antibody
      • Cerb B2/neu protein antibody
      • CerbB2 antibody
      • Erb b2 receptor tyrosine kinase 2 antibody
      • ERBB2 antibody
      • ERBB2_HUMAN antibody
      • HER 2 antibody
      • HER 2/NEU antibody
      • HER2 antibody
      • Herstatin antibody
      • Human epidermal growth factor receptor 2 antibody
      • Metastatic lymph node gene 19 protein antibody
      • MLN 19 antibody
      • MLN19 antibody
      • NEU antibody
      • NEU proto oncogene antibody
      • Neuro/glioblastoma derived oncogene homolog antibody
      • Neuroblastoma/glioblastoma derived oncogene homolog antibody
      • NGL antibody
      • p185erbB2 antibody
      • Proto-oncogene c-ErbB-2 antibody
      • Proto-oncogene Neu antibody
      • Receptor tyrosine-protein kinase erbB-2 antibody
      • TKR1 antibody
      • Tyrosine kinase type cell surface receptor HER2 antibody
      • Tyrosine kinase-type cell surface receptor HER2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      see all

    Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] images

    • All lanes : Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/500 dilution

      Lane 1 : A431 whole cell lysate - untreated
      Lane 2 : A431 whole cell lysate - treated with Epidermal Growth Factor (EGF)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 138 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of ErbB 2 (pY1139) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/1000 dilution

      Lane 1 : SKBR3 cell lysate
      Lane 2 : SKBR3 cell lysate - treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 138 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/2000 dilution

      Lane 1 : HTB-2O Human breast ductal carcinoma lysate untreated
      Lane 2 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 3 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 4 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 5 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 6 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 7 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 8 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
      Lane 9 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated Mouse anti-Rabbit monoclonal at 1/20000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 138 kDa


      Exposure time : 5 seconds

      This image is courtesy of an Abreview by Surekha Pimple.

      Lanes 2-9 are treated with an increasing concentration of HER2 inhibitor.
      Blocking performed with 5% Milk for 1 hour at room temperature.
      Antibody diluted in CPPT and incubated for 16 hours at 4°C.
      Performed under denaturing conditions.

      See Abreview

    References for Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] (ab53290)

    This product has been referenced in:
    • Patel N  et al. The growth factor receptor ERBB2 regulates mitochondrial activity on a signaling time scale. J Biol Chem 288:35253-65 (2013). WB . Read more (PubMed: 24142693) »
    • Zhang Q  et al. Functional isolation of activated and unilaterally phosphorylated heterodimers of ERBB2 and ERBB3 as scaffolds in ligand-dependent signaling. Proc Natl Acad Sci U S A 109:13237-42 (2012). WB . Read more (PubMed: 22733765) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HTB-2O Human breast ductal carcinoma)
    Loading amount 10 µg
    Specification HTB-2O Human breast ductal carcinoma
    Treatment Untreated /treated with HER-2 inhibitor
    Gel Running Conditions Reduced Denaturing (9 % SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mrs. surekha pimple

    Verified customer

    Submitted Nov 26 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"