Overview

  • Product nameAnti-ErbB 3 antibody
    See all ErbB 3 primary antibodies
  • Description
    Rabbit polyclonal to ErbB 3
  • SpecificityThe antibody is directed against human HER3. experiments.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    HER3 fusion protein:

    PASEQGYE E MRAFQGPGH QAPHVHYARL KTLRSLEAT D SAF

    , corresponding to C terminal amino acids 1283-1323 of Human HER3

  • Positive control
    • Human prostate cell lines (LNCap) or human breast cell line (MDA-453)

Properties

Applications

Our Abpromise guarantee covers the use of ab34641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 5µg. The immunoprecipitation mix contained the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).

WB Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 148 kDa).

In conjunction with a detection antibody like anti-phosphotyrosine.

Target

  • FunctionBinds and is activated by neuregulins and NTAK.
  • Tissue specificityEpithelial tissues and brain.
  • Involvement in diseaseDefects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageOverexpressed in a subset of human mammary tumors.
  • DomainThe cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • Post-translational
    modifications
    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c erbB 3 antibody
    • c erbB3 antibody
    • Erb b2 receptor tyrosine kinase 3 antibody
    • ErbB 3 antibody
    • ERBB3 antibody
    • ERBB3 protein antibody
    • erbB3 S antibody
    • ERBB3_HUMAN antibody
    • Glial growth factor receptor antibody
    • HER 3 antibody
    • HER3 antibody
    • Human epidermal growth factor receptor 3 antibody
    • LCCS2 antibody
    • MDA BF 1 antibody
    • MGC88033 antibody
    • p180 ErbB3 antibody
    • p45 sErbB3 antibody
    • p85 sErbB3 antibody
    • proto-oncogene-like protein c ErbB 3 antibody
    • proto-oncogene-like protein c ErbB3 antibody
    • Proto-oncogene-like protein c-ErbB-3 antibody
    • Receptor tyrosine protein kinase erbB 3 antibody
    • Receptor tyrosine protein kinase erbB3 antibody
    • Receptor tyrosine-protein kinase erbB-3 antibody
    • Tyrosine kinase type cell surface receptor HER3 antibody
    • Tyrosine kinase-type cell surface receptor HER3 antibody
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
    see all

Anti-ErbB 3 antibody images

  • All lanes : Anti-ErbB 3 antibody (ab34641) at 5 µl (for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody. )

    Lane 1 : a human LNCap prostate cell line without the additon of Heregulin ß1
    Lane 2 : a human LNCap prostate cell line treatment with Heregulin ß1 for 15' at 100 ng/ml

    Developed using the ECL technique

    Predicted band size : 148 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

References for Anti-ErbB 3 antibody (ab34641)

This product has been referenced in:
  • Yan X  et al. miR-143 and miR-145 synergistically regulate ERBB3 to suppress cell proliferation and invasion in breast cancer. Mol Cancer 13:220 (2014). WB ; Human . Read more (PubMed: 25248370) »

See 1 Publication for this product

Product Wall


The Anti-ErbB 3 antibody (ab34641) has been raised against a fusion protein corresponding to the C-terminal residues of human HER3 (residues 1283-1323). We do not have this protein available for purchase.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"