Our Abpromise guarantee covers the use of ab34641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 5µg. The immunoprecipitation mix contained the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).
|WB||Use at an assay dependent concentration. Detects a band of approximately 180 kDa (predicted molecular weight: 148 kDa).
In conjunction with a detection antibody like anti-phosphotyrosine.