Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] (ab76469)

Overview

  • Product nameAnti-ErbB 3 (phospho Y1289) antibody [EPR2325]
    See all ErbB 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR2325] to ErbB 3 (phospho Y1289)
  • Specificityab76469 only detects ErbB 3 phosphorylated on Tyrosine 1289.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Tyrosine 1289 of Human ErbB 3

  • Positive control
    • SKBR3 cell lysate treated with Neuregulin. MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
ICC/IF 1/100.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionBinds and is activated by neuregulins and NTAK.
    • Tissue specificityEpithelial tissues and brain.
    • Involvement in diseaseDefects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Developmental stageOverexpressed in a subset of human mammary tumors.
    • DomainThe cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
    • Post-translational
      modifications
      Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] images

    • Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.
    • All lanes : Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution

      Lane 1 : SKBR3 cell lysate untreated
      Lane 2 : SKBR3 cell lysate treated with Neuregulin

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 148 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)

    References for Anti-ErbB 3 (phospho Y1289) antibody [EPR2325] (ab76469)

    This product has been referenced in:
    • Humtsoe JO  et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). Read more (PubMed: 26073080) »
    • Sollome JJ  et al. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal 26:70-82 (2013). Human . Read more (PubMed: 24036211) »

    See all 3 Publications for this product

    Product Wall

    ab51044
    -Note yet tested on frozen sections but has been tested on paraffin embedded sections
    fixation methods: formalin
    Recommended starting dilution: 1:50
    Positive control: testis, brain
    Negative control: protein is widely ex...

    Read More

    Thank you for your enquiry. Unfortunately, we do not have a fluorescence conjugated version of this product. Also, a custom BSA-free production is not available.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"