Overview

  • Product nameAnti-ErbB 4 antibody
    See all ErbB 4 primary antibodies
  • Description
    Rabbit polyclonal to ErbB 4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 - 1150 of Human ErbB 4.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Y79; MOLT4; SKNBE; MCF7; LNCaP; HepG2; HEK293. This antibody gave a positive signal in the following FFPE tissue: Human normal hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab113246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).

Target

  • FunctionSpecifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
  • Tissue specificityExpressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
    Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
    Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
  • Cellular localizationMembrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4ICD antibody
    • ALS19 antibody
    • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
    • E4ICD antibody
    • EC 2.7.10.1 antibody
    • Erbb4 antibody
    • ERBB4 intracellular domain antibody
    • ERBB4_HUMAN antibody
    • HER 4 antibody
    • HER4 antibody
    • human epidermal growth factor receptor 4 antibody
    • Mer4 antibody
    • MGC138404 antibody
    • Oncogene ERBB4 antibody
    • p180erbB4 antibody
    • Proto-oncogene-like protein c-ErbB-4 antibody
    • Receptor protein tyrosine kinase erbB 4 precursor antibody
    • Receptor tyrosine protein kinase erbB 4 antibody
    • s80HER4 antibody
    • Tyrosine kinase type cell surface receptor HER4 antibody
    • Tyrosine kinase-type cell surface receptor HER4 antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
    • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
    • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
    • VERBB2 antibody
    see all

Anti-ErbB 4 antibody images

  • All lanes : Anti-ErbB 4 antibody (ab113246) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : LNCaP human prostate carcinoma cell line lysate Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 147 kDa
    Observed band size : 147 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • IHC image of ErbB 4 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ErbB 4 antibody (ab113246)

ab113246 has not yet been referenced specifically in any publications.

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