Overview

  • Product nameErbB2 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.19 pg/ml - 2000 pg/ml
  • Recovery

    > 81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.77 70% - 96%
    Serum 91.93 76% - 106%
    Plasma 81.32 69% - 97%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ErbB2 (Epidermal Factor Growth Factor Receptor 2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human ErbB2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
  • Alternative names
    • c erb B2
    • C erbB 2
    • CD340
    • CD340 antigen
    • Cerb B2/neu protein
    • CerbB2
    • Erb B2
    • erbb2
    • HER 2
    • HER 2/neu
    • HER2
    • Her2/neu
    • Herstatin
    • Metastatic lymph node gene 19 protein
    • MLN 19
    • MLN19
    • NEU
    • NEU Proto Oncogene
    • Neuro Glioblastoma Derived Oncogene Homolog
    • Neuroblastoma/glioblastoma derived oncogene homolog
    • NGL
    • p185 ErbB2
    • p185erbB2
    • Proto oncogene c ErbB 2
    • Proto oncogene Neu
    • Receptor protein tyrosine kinase ErbB2 precursor
    • Receptor tyrosine protein kinase erbB 2
    • TKR1
    • Tyrosine kinase type cell surface receptor HER2
    • v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian)
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ErbB2 Human ELISA Kit images

  • Representative Standard Curve using ab100510.

  • Representative Standard Curve using ab100510.

Protocols

References for ErbB2 Human ELISA Kit (ab100510)

ab100510 has not yet been referenced specifically in any publications.

Product Wall

HER2 ELISA KIT used for testing HER2 in the urine

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We wanted to measure HER2 in the urine, using the Abcam HER2 ELISA kit. We did not dilute the urine samples and we were able to detect the levels of the protein using the kit. The concentrations of HER2 in the urine of healthy individuals are really low (below 100 pg/ml), so the sensitivity of the kit is a major issue. The Abcam HER2 ELISA kit had a better sensitivity than other kits available in the market.
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Abcam user community

Verified customer

Submitted Dec 09 2013

Thank you very much for your interest in ab100510.

To our knowledge, ab100510 has not been tested in monkey samples, howvever there is a 97% level of homology between the human and monkey sequences, therefore there is a a good chance the kit w...

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The capture antibody is monoclonal and from mouse, while the detection antibody is polyclonal and from goat. Both antibodies detect the extracellular domain of ErbB2.

I hope this is helpful. Please let me know if you need further assistance.

Thank you for contacting us.  Yes, the customer could certainly use the included antibody and reagents to detect ErbB 2 in a test sample that has been directly coated to an empty ELISA plate.  If the customer has the detection reagents, he or she could...

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Thank you for contacting us. The standard provided with this kit corresponds to amino acids Thr23 - Thr652, which is the extracellular domain of ErbB2. Unfortunately, the antibodies for this kit are not currently available to purchase separate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"