Overview

  • Product nameErbB3 Human ELISA kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    3.43 pg/ml - 2500 pg/ml
  • Recovery

    > 90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.1 78% - 113%
    Serum 95.12 79% - 111%
    Plasma 88.87 71% - 106%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ErbB3 (Epidermal Factor Growth Factor Receptor 3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human ErbB3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceThe ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.
  • Alternative names
    • ERBB3
    • HER3
    • Proto oncogene like protein cErbB3
    • Receptor tyrosine protein kinase erbB3
    • Tyrosine kinase type cell surface receptor HER3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ErbB3 Human ELISA kit images

  • TYPICAL STANDARD CURVE - Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
  • TYPICAL STANDARD CURVE - Assay Dilent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References for ErbB3 Human ELISA kit (ab100511)

ab100511 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry and sending that information. I went ahead and plotted the data using a linear regression model and it looks beautiful with strong signal strength, low duplicate CV, and nice linearity (R2 > 0.99). See attachment. The manu...

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Thank you for contacting Abcam. It was a pleasure speaking with you. I have a response from the lab to the the questions that you had brought up yesterday. In regards to whether the antibody works against the free or bound forms of ErbB3, the ELH-ErbB3...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"