The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionActs as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.
Tissue specificityHighly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.
Sequence similaritiesBelongs to the LAP (LRR and PDZ) protein family. Contains 17 LRR (leucine-rich) repeats. Contains 1 PDZ (DHR) domain.
Cellular localizationCell junction > hemidesmosome. Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane.