The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 157 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionActs as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.
Tissue specificityHighly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.
Sequence similaritiesBelongs to the LAP (LRR and PDZ) protein family. Contains 17 LRR (leucine-rich) repeats. Contains 1 PDZ (DHR) domain.
Cellular localizationCell junction > hemidesmosome. Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane.
Pan P & Dobrowsky RT Differential expression of neuregulin-1 isoforms and downregulation of erbin are associated with Erb B2 receptor activation in diabetic peripheral neuropathy. Acta Neuropathol Commun1:39 (2013).
Read more (PubMed: 24252174) »
Lipinski S et al. RNAi screening identifies mediators of NOD2 signaling: Implications for spatial specificity of MDP recognition. Proc Natl Acad Sci U S A : (2012).
Read more (PubMed: 23213202) »