Overview

  • Product nameAnti-ERC2 antibody
    See all ERC2 primary antibodies
  • Description
    Rabbit polyclonal to ERC2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Rat ERC2.

    (Peptide available as ab27861.)

  • Positive control
    • This antibody gave a positive signal in Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab27250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionThought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ.
  • Cellular localizationCytoplasm. Cell junction > synapse. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. In neurons, localized to synapses, and colocalizes with PCLO. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cast antibody
    • CAST1 antibody
    • CAZ-associated structural protein 1 antibody
    • Cmbp (gene name) antibody
    • Cytomatrix protein p110 antibody
    • ERC protein 2 antibody
    • Erc2 antibody
    • ERC2_HUMAN antibody
    • KIAA0378 antibody
    see all

Anti-ERC2 antibody images

  • Anti-ERC2 antibody (ab27250) at 1 µg/ml + Rat Brain Whole Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 111 kDa
    Observed band size : 111 kDa
    Additional bands at : 50 kDa (possible non-specific secondary antibody binding).
  • Image courtesy of Human Protein Atlas

    ab27250 staining ERC2 in Human cerebellum. The paraffin embedded human tissue was incubated with ab27250 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab27250 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

References for Anti-ERC2 antibody (ab27250)

ab27250 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"