• Product nameAnti-ERCC1 antibody [EP2143Y]
    See all ERCC1 primary antibodies
  • Description
    Rabbit monoclonal [EP2143Y] to ERCC1
  • Specificityab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ERCC1 aa 1-100 (N terminal).

  • Positive control
    • SKBR-3 cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionStructure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
    • Involvement in diseaseDefects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    • Sequence similaritiesBelongs to the ERCC1/RAD10/SWI10 family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • COFS 4 antibody
      • COFS4 antibody
      • DNA excision repair protein ERCC 1 antibody
      • DNA excision repair protein ERCC-1 antibody
      • DNA excision repair protein ERCC1 antibody
      • ERCC 1 antibody
      • ERCC1 antibody
      • ERCC1_HUMAN antibody
      • Excision repair cross complementation group 1 antibody
      • Excision repair cross complementing 1 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
      • Excision repair protein antibody
      • RAD 10 antibody
      • RAD10 antibody
      • UV 20 antibody
      • UV20 antibody
      see all

    Anti-ERCC1 antibody [EP2143Y] images

    • Anti-ERCC1 antibody [EP2143Y] (ab76236) at 1/2000 dilution + SKBR-3 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-ERCC1 antibody [EP2143Y] (ab76236)

    ab76236 has not yet been referenced specifically in any publications.

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