Overview

  • Product nameAnti-ERCC6L antibody
    See all ERCC6L primary antibodies
  • Description
    Mouse monoclonal to ERCC6L
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-420 of Human FLJ20105 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab57434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 TPR repeats.
    • Post-translational
      modifications
      Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
    • Cellular localizationChromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent helicase ERCC6 like antibody
      • ATP-dependent helicase ERCC6-like antibody
      • DNA excision repair protein ERCC 6 like antibody
      • DNA excision repair protein ERCC-6-like antibody
      • ERC6L_HUMAN antibody
      • ERCC 6L antibody
      • Ercc6l antibody
      • Excision repair cross complementing rodent repair deficiency complementation group 6 like antibody
      • Excision repair protein ERCC6 like antibody
      • FLJ20105 antibody
      • MGC131695 antibody
      • PICH antibody
      • Plk1 interacting checkpoint helicase antibody
      • PLK1-interacting checkpoint helicase antibody
      • SNF2/RAD54 family protein antibody
      • Tumor antigen BJ HCC 15 antibody
      • Tumor antigen BJ-HCC-15 antibody
      see all

    Anti-ERCC6L antibody images

    • Western blot against tagged recombinant protein immunogen using ab57434 FLJ20105 protein antibody at 1ug/ml. Predicted band size of immunogen is 72 kDa

    References for Anti-ERCC6L antibody (ab57434)

    ab57434 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"