Overview

  • Product name
  • Description
    Rabbit polyclonal to ERF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 1 and 265 of Human ERF

  • Positive control
    • A431 cell lysate; Paraffin-embedded U373 xenograft; Paraformaldehyde-fixed HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab153726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • Tissue specificity
    Highest levels in testis, ovary, pancreas, and heart.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Images

  • Anti-ERF antibody (ab153726) at 1/1000 dilution + A431 cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded U373 xenograft, labeling ERF using ab153726 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling ERF using ab153726 at 1/500 dilution. Right panel costained with Hoechst 33342.

References

ab153726 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153726.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up