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Rabbit polyclonal to ERF
Predicted to work with:
Mouse, Rat, Cow
A recombinant fragment corresponding to a region within amino acids 1 and 265 of Human ERF
A431 cell lysate; Paraffin-embedded U373 xenograft; Paraformaldehyde-fixed HeLa cells
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
1/100 - 1/1000.
1/100 - 1/1000.
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
Highest levels in testis, ovary, pancreas, and heart.
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
Information by UniProt
ETS domain containing transcription factor ERF antibody
Western blot - Anti-ERF antibody (ab153726)
Anti-ERF antibody (ab153726) at 1/1000 dilution + A431 cell lysate at 30 µg
Predicted band size: 59 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (ab153726)
Immunohistochemical analysis of paraffin-embedded U373
xenograft, labeling ERF using ab153726 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ERF antibody (ab153726)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling ERF using ab153726 at 1/500 dilution. Right panel costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"