The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
FunctionPotent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
Tissue specificityHighest levels in testis, ovary, pancreas, and heart.
Sequence similaritiesBelongs to the ETS family. Contains 1 ETS DNA-binding domain.
Post-translational modificationsPhosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
Immunofluorescence analysis of HeLa cells, using ab62612 at 1:500 dilution.
Left image untreated.
Right image treated with peptide.
References for Anti-ERF antibody (ab62612)
This product has been referenced in:
Fu H et al. A genome-wide screen for spatially restricted expression patterns identifies transcription factors that regulate glial development. J Neurosci29:11399-408 (2009).
Read more (PubMed: 19741146) »