Overview

  • Product nameAnti-ERF antibody
    See all ERF primary antibodies
  • Description
    Rabbit polyclonal to ERF
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 498 and 548 of human ERF (NP_006485.2).

  • Positive control
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma Mouse Controls: Renal Cell Carcinoma, Teratoma

Properties

Applications

Our Abpromise guarantee covers the use of ab84773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPotent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
    • Tissue specificityHighest levels in testis, ovary, pancreas, and heart.
    • Sequence similaritiesBelongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERF antibody
      • ERF_HUMAN antibody
      • ETS domain containing transcription factor ERF antibody
      • ETS domain-containing transcription factor ERF antibody
      • Ets2 repressor factor antibody
      • PE 2 antibody
      • PE-2 antibody
      • PE2 antibody
      see all

    Anti-ERF antibody images

    • Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of human ovarian tumor using a 1/100 dilution of ab84773. Detection: DAB staining
    • Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/500 dilution of ab84773. Detection: DAB staining

    References for Anti-ERF antibody (ab84773)

    ab84773 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"