Overview

  • Product nameAnti-ERF antibody
    See all ERF primary antibodies
  • Description
    Rabbit polyclonal to ERF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 360-409 ((VPSSASSSSS SSSSPFKFKL QRPPLGRRQR AAGEKAVAAA DKSGGSAGGL) of human ERF (AAA86686).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPotent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • Tissue specificityHighest levels in testis, ovary, pancreas, and heart.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Anti-ERF antibody images

  • Anti-ERF antibody (ab87039) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : ~59 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ERF antibody (ab87039)

ab87039 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87039.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"