Anti-ERF (phospho T526) antibody (ab59178)

Overview

  • Product nameAnti-ERF (phospho T526) antibody
    See all ERF primary antibodies
  • Description
    Rabbit polyclonal to ERF (phospho T526)
  • SpecificityDetects endogenous levels of ERF only when phosphorylated at threonine 526.
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of threonine 526 (PLTPPR) of human ERF

  • Positive control
    • Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab59178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionPotent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
  • Tissue specificityHighest levels in testis, ovary, pancreas, and heart.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERF antibody
    • ERF_HUMAN antibody
    • ETS domain containing transcription factor ERF antibody
    • ETS domain-containing transcription factor ERF antibody
    • Ets2 repressor factor antibody
    • PE 2 antibody
    • PE-2 antibody
    • PE2 antibody
    see all

Anti-ERF (phospho T526) antibody images

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59178 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.

References for Anti-ERF (phospho T526) antibody (ab59178)

ab59178 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 35 µg
Specification HEK293 cells
Treatment 100ng/ml nocodazole 6 hours
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"