Overview

  • Product name
  • Description
    Rabbit polyclonal to eRF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human eRF1 aa 388-437 (C terminal). The exact sequence is proprietary.

  • Positive control
    • 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates; SW480 xenograft.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-eRF1 antibody (ab153731) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded SW480 xenograft labeling eRF1 using ab153731 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling eRF1 using ab153731 at 1/200 dilution.
    The image in the right panel was merged with DNA probe.

References

ab153731 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153731.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up