This antibody gave a positive control in the following human lysates:
HeLa (Human epithelial carcinoma cell line) whole cell
Jurkat (Human T cell lymphoblast-like cell line) whole cell
A431 (Human epithelial carcinoma cell line) whole cell
This antibody gave a positive control in the following mouse lysates:
NIH 3T3 whole cell
MEF1 whole cell
This antibody gave a positive control in the following rat lysate:
PC12 whole cell
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/35. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Use a concentration of 1 µg/ml.
FunctionDirects the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Sequence similaritiesBelongs to the eukaryotic release factor 1 family.
ICC/IF image of ab30928 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30928, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas ab30928 staining eRF1 in human gall bladder, showing a cytoplasmic staining pattern in glandular cells. Paraffin embedded human gall bladder tissue was incubated with ab30928 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab30928 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-eRF1 antibody (ab30928)
This product has been referenced in:
Zhai Y et al. Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation. Mol Cell Biol28:7414-26 (2008).
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