This antibody gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
This antibody gave a positive result in the following tissue lysates:
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Use a concentration of 1 µg/ml.
Use at an assay dependent dilution. PubMed: 20606008
FunctionDirects the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Sequence similaritiesBelongs to the eukaryotic release factor 1 family.
ICC/IF image of ab31799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31799, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-eRF1 antibody (ab31799)
This product has been referenced in:
Liu P et al. Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality. Mol Cell Biol30:4245-53 (2010).
Read more (PubMed: 20606008) »