Overview

  • Product nameAnti-eRF3B/GSPT2 antibody
    See all eRF3B/GSPT2 primary antibodies
  • Description
    Rabbit polyclonal to eRF3B/GSPT2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 143-192 (MALEESWEHSKEVSEAEPGGGSSGDSGPPEESGQEMMEEKEEIRKSKSV I) of Human eRF3B/GSPT2 (NP_060564)

  • Positive control
    • MCF7 cytoplasmic lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
  • Tissue specificityHighly expressed in IUCC stage II colorectal cancer (CRC).
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. ERF3 subfamily.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eRF3b antibody
    • ERF3B_HUMAN antibody
    • Eukaryotic peptide chain release factor GTP-binding subunit ERF3B antibody
    • Eukaryotic peptide chain release factor subunit 3b antibody
    • G1 to S phase transition 2 antibody
    • G1 to S phase transition protein 2 homolog antibody
    • Gspt2 antibody
    • GST2 antibody
    • peptide chain release factor 3 antibody
    see all

Anti-eRF3B/GSPT2 antibody images

  • Anti-eRF3B/GSPT2 antibody (ab84180) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

References for Anti-eRF3B/GSPT2 antibody (ab84180)

ab84180 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"