Overview

  • Product name
  • Description
    Rabbit polyclonal to eRF3/GSPT1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-347 of Human eRF3/GSPT1 (NP_002085).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; Human Breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 551G9.2 antibody
    • Anti Eukaryotic Release Factor 3a antibody
    • Anti G1 to S phase transition 1 antibody
    • Anti GST1, homolog of yeast antibody
    • eRF 3a antibody
    • eRF3a antibody
    • G1 to S phase transition 1 antibody
    • G1 to S phase transition protein 1 homolog antibody
    • G1 to S phase transition protein antibody
    • GSPT 1 antibody
    • GSPT1 antibody
    • GST 1 antibody
    • GST1 antibody
    • GTP binding protein GST1 HS antibody
    • Transition protein 1 antibody
    see all

Images

  • Anti-eRF3/GSPT1 antibody (ab126090) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 69 kDa
  • ab126090 at 1/500 dilution, staining eRF3/GSPT1 in Paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

References

ab126090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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