Anti-eRF3/GSPT1 antibody (ab49878)
Key features and details
- Rabbit polyclonal to eRF3/GSPT1
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-eRF3/GSPT1 antibody
See all eRF3/GSPT1 primary antibodies -
Description
Rabbit polyclonal to eRF3/GSPT1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:FETGFEKGGQTREHA
Database link: P15170 -
Positive control
- WB: HeLa whole cell lysate (ab150035).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
Paraformadehyde fixed cells |
|
WB | (2) |
Use at an assay dependent concentration.
|
Notes |
---|
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. Paraformadehyde fixed cells |
WB
Use at an assay dependent concentration. |
Target
-
Function
Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.
Contains 1 tr-type G (guanine nucleotide-binding) domain. - Information by UniProt
-
Database links
- Entrez Gene: 2935 Human
- Omim: 139259 Human
- SwissProt: P15170 Human
- Unigene: 528780 Human
-
Alternative names
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab49878 has been referenced in 15 publications.
- Lee RE et al. Small-molecule eRF3a degraders rescue CFTR nonsense mutations by promoting premature termination codon readthrough. J Clin Invest 132:N/A (2022). PubMed: 35900863
- Powell CE et al. Selective degradation-inducing probes for studying cereblon (CRBN) biology. RSC Med Chem 12:1381-1390 (2021). PubMed: 34458741
- Shimizu N et al. PLZF and its fusion proteins are pomalidomide-dependent CRBN neosubstrates. Commun Biol 4:1277 (2021). PubMed: 34764413
- Tateno S et al. Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide. Sci Rep 10:4012 (2020). PubMed: 32132601
- Powell CE et al. Selective Degradation of GSPT1 by Cereblon Modulators Identified via a Focused Combinatorial Library. ACS Chem Biol 15:2722-2730 (2020). PubMed: 32865967