Recombinant
RabMAb

Anti-ERG antibody [EPR3863] - ChIP Grade (ab110639)

Overview

  • Product name
    Anti-ERG antibody [EPR3863] - ChIP Grade
    See all ERG primary antibodies
  • Description
    Rabbit monoclonal [EPR3863] to ERG - ChIP Grade
  • Specificity
    ab110639 also detects Fli1.
  • Tested applications
    Suitable for: WB, IHC-P, ChIPmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to residues on the N-terminus in Human ERG.

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab110639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration. PubMed: 22615383
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
    • Involvement in disease
      Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
      Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
    • Sequence similarities
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Cellular localization
      Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
      • D030036I24Rik antibody
      • Erg 3 antibody
      • Erg antibody
      • ERG/EWS fusion gene, included antibody
      • ERG/FUS fusion gene, included antibody
      • ERG/TMPSSR2 fusion gene, included antibody
      • ERG_HUMAN antibody
      • ERG1, included antibody
      • ERG2, included antibody
      • ets related antibody
      • ETS-related gene antibody
      • KCNH2 antibody
      • Oncogene ERG antibody
      • p55 antibody
      • TMPRSS2/ERG fusion antibody
      • transcriptional regulator ERG (transforming protein ERG) antibody
      • Transcriptional regulator ERG antibody
      • Transforming protein ERG antibody
      • v ets avian erythroblastosis virus E26 oncogene antibody
      • v ets avian erythroblastosis virus E26 oncogene related antibody
      • v ets erythroblastosis virus E26 oncogene homolog antibody
      • v ets erythroblastosis virus E26 oncogene like antibody
      • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
      • v-ets erythroblastosis virus E26 oncogene antibody
      • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
      • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
      see all

    Images

    • All lanes : Anti-ERG antibody [EPR3863] - ChIP Grade (ab110639) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
    • ab110639 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
    • ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    References

    This product has been referenced in:
    • Rickman DS  et al. Oncogene-mediated alterations in chromatin conformation. Proc Natl Acad Sci U S A 109:9083-8 (2012). ChIP . Read more (PubMed: 22615383) »
    • Mohamed AA  et al. Ets family protein, erg expression in developing and adult mouse tissues by a highly specific monoclonal antibody. J Cancer 1:197-208 (2010). WB ; Human . Read more (PubMed: 21060730) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E10.5)
    Specification
    E10.5
    Username

    Dr. Sophie Astrof

    Verified customer

    Submitted Jan 31 2017

    Thank you very much for your interest in ab110639.

    To our knowledge,ab110639 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab110639 now, test it inMouse and submit feedback to us in the form...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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