Overview

  • Product nameAnti-ERG antibody [EPR3864]
    See all ERG primary antibodies
  • Description
    Rabbit monoclonal [EPR3864] to ERG
  • SpecificityThis antibody also detects Fli-1.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Fr, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ERG aa 450 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: Jurkat cell lysate. IHC: Human prostate adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-ERG antibody (HRP) [EPR3864] (ab195514)

    Anti-ERG antibody (Alexa Fluor® 488) [EPR3864] (ab196374)

    Anti-ERG antibody (Alexa Fluor® 647) [EPR3864] (ab196149)

Properties

Applications

Our Abpromise guarantee covers the use of ab92513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified, use 1/100 - 1/250.

ICC/IF 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 22860005
ChIP Use at an assay dependent concentration. PubMed: 23817021

Target

  • FunctionTranscriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Involvement in diseaseDefects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
    • D030036I24Rik antibody
    • Erg 3 antibody
    • Erg antibody
    • ERG/EWS fusion gene, included antibody
    • ERG/FUS fusion gene, included antibody
    • ERG/TMPSSR2 fusion gene, included antibody
    • ERG_HUMAN antibody
    • ERG1, included antibody
    • ERG2, included antibody
    • ets related antibody
    • ETS-related gene antibody
    • KCNH2 antibody
    • Oncogene ERG antibody
    • p55 antibody
    • TMPRSS2/ERG fusion antibody
    • transcriptional regulator ERG (transforming protein ERG) antibody
    • Transcriptional regulator ERG antibody
    • Transforming protein ERG antibody
    • v ets avian erythroblastosis virus E26 oncogene antibody
    • v ets avian erythroblastosis virus E26 oncogene related antibody
    • v ets erythroblastosis virus E26 oncogene homolog antibody
    • v ets erythroblastosis virus E26 oncogene like antibody
    • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
    • v-ets erythroblastosis virus E26 oncogene antibody
    • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
    • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
    see all

Anti-ERG antibody [EPR3864] images

  • All lanes : purified

    Lane 1 : rat brain lysate
    Lane 2 : rat heart lysate
    Lane 3 : Raw264.7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-ERG antibody [EPR3864] (ab92513) at 1/2000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of THP-1 cells with purified ab92513 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92513 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab92513 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ab92513 staining ERG in Human brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 3% Horse serum) for 15 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size : 55 kDa
  • Lanes 1 & 3 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (unpurified)
    Lanes 2 & 4 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 12 minutes
  • Immunohistochemical analysis of Fus A5 transgenic mouse prostate tissue, staining ERG with unpurified ab92513.

    Tissue sections were blocked in 20% goat serum, 2% BSA in PBS (GSB) for 40 min at room temperature, followed by an overnight incubation in primary antibody (1/25 in GSB) at 4°C. Following washes, the sections were incubated with a fluorescently tagged secondary antibody for 30 min at room temperature.

  • Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using unpurified ab92513 showing +ve staining.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-ERG antibody [EPR3864] (ab92513)

This product has been referenced in:
  • Zhang C  et al. Catalpol downregulates vascular endothelial-cadherin expression and induces vascular hyperpermeability. Mol Med Rep 13:373-8 (2016). WB . Read more (PubMed: 26549479) »
  • Pippin JW  et al. Cells of renin lineage are adult pluripotent progenitors in experimental glomerular disease. Am J Physiol Renal Physiol 309:F341-58 (2015). ICC/IF ; Mouse . Read more (PubMed: 26062877) »

See all 22 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Application IHC - Wholemount
Sample Mouse Tissue (whole retina)
Specification whole retina
Username

Mr. Nicolas Rama

Verified customer

Submitted Nov 17 2014

We looked into this about 3 years ago when one of our customers reported that ab110639 and ab92513 would recognize Fli-1. Her data demonstrated that immunoprecipitation with ab92513 and mass-spec sequencing from the CMK cell line immunoprecipitates the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Vascular tissue)
Specification Vascular tissue
Permeabilization Yes - 0.05% Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 30 2013

Thank you for contacting us.

The concentration of current lot GR106923-3 is XXXXX mg/ml.

Please note the rabbit monoclonal antibodies are more specific than their mouse and rabbit counter parts which is why you may find the concentrat...

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Thank you for your inquiry.
These two ERG antibodies are raised against the same immunogen but they are different subclones. The clone numbers are
slightly different.
This is why the specificity, species reactivity and recommended applica...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"