Overview

  • Product name
  • Description
    Rabbit polyclonal to ERGIC1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PGNFHVSTHS ATAQPQNPDM THVIHKLSFG DTLQVQNIHG AFNALGGADR LTSNPLASHD YIL, corresponding to internal sequence amino acids 127-189 of Human ERGIC1

  • Positive control
    • Human colon tissue; transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag.

Properties

Applications

Our Abpromise guarantee covers the use of ab121582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

Target

  • Function
    Possible role in transport between endoplasmic reticulum and Golgi.
  • Sequence similarities
    Belongs to the ERGIC family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
    • Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
    • ER-Golgi intermediate compartment 32 kDa protein antibody
    • ERGI1_HUMAN antibody
    • ERGIC-32 antibody
    • ergic1 antibody
    • ERGIC32 antibody
    • KIAA1181 antibody
    • NET24 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.
  • All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T cell lysate
    Lane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique
  • Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582

References

ab121582 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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