The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPossible role in transport between endoplasmic reticulum and Golgi.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the ERGIC family.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cytoplasm. Nucleus. Cycles between the endoplasmic reticulum and the Golgi. PubMed:11445006 states that it localizes to the nucleus; PubMed:17980171 shows a partial localization in the nucleus.