Overview

  • Product nameAnti-ERK1 + ERK2 antibodySee all ERK1 + ERK2 primary antibodies ...
  • Description
    Sheep polyclonal to ERK1 + ERK2
  • Tested applicationsIPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant p42/p44 MAP kinase protein.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 1 - 10 µg/ml.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p42MAPK antibody
    • p42MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44ERK1 antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • p44MAPK antibody
    • PRKM 1 antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

References for Anti-ERK1 + ERK2 antibody (ab16573)

ab16573 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 8e+006 cells
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Sample Human Cell lysate - whole cell (Human washed platelets)
Specification Human washed platelets
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"