Overview

  • Product nameAnti-ERK1 + ERK2 antibody
    See all ERK1 + ERK2 primary antibodies
  • Description
    Rabbit polyclonal to ERK1 + ERK2
  • Tested applicationsIHC-P, ICC, ICC/IF, IP, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, Culex pipiens
  • Immunogen

    Synthetic peptide corresponding to Human ERK1 + ERK2 aa 317-339 (C terminal).
    Sequence:

    RIT VEEALAHPYL EQYYDPTDE


    Database link: P27361

  • Positive control
    • A431, PC12, and NIH3T3 cells.
  • General notes

    Please note that this is an intracellular epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab17942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
ICC 1/200.
ICC/IF 1/200.
IP Use at 1 µg/mg of lysate.
IHC-Fr 1/200.
WB 1/1000. Predicted molecular weight: 42-44 kDa.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p42MAPK antibody
    • p42MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44ERK1 antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • p44MAPK antibody
    • PRKM 1 antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

Anti-ERK1 + ERK2 antibody images



  • Predicted band size : 42-44 kDa

    Western Blot for ab17942.

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
    with goat anti-rabbit IgG alkaline phosphatase.

    These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.

     

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-ra

  • All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

    Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
    Lane 2 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
    Lane 3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42-44 kDa
    Observed band size : 42,44 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X, PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in Embryonic Stem Cell-induced Mouse Neurospheres by Immunohistochemistry (Frozen sections). Sections were formaldehyde-fixed prior to blocking in 10% serum for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal antibody, diluted 1/1500 was used as the secondary.

    See Abreview

  • ab17942 at 1/200 staining human (ab30203), rat (ab29480) and mouse (ab7261) kidney sections by IHC-P. The tissue sections were formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody.

    See Abreview

  • ab17942 staining ERK1 + ERK2 in AGS human gastric carcinoma cells by Immunocytochemistry. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X/ PBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with the primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to HRP, used undiluted.

    See Abreview

References for Anti-ERK1 + ERK2 antibody (ab17942)

This product has been referenced in:
  • Branham-O'Connor M  et al. Defective chemokine signal integration in leukocytes lacking activator of G protein signaling 3 (AGS3). J Biol Chem 289:10738-47 (2014). WB ; Mouse . Read more (PubMed: 24573680) »
  • Turner ML  et al. Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6. Endocrinology 155:1453-65 (2014). WB ; Cow . Read more (PubMed: 24437488) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification whole fly homogenates
Treatment 30 minutes after blood feeding with sheep blood
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Jiawen Xu

Verified customer

Submitted Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (stomach cancer)
Loading amount 30 µg
Specification stomach cancer
Treatment 10uM WIN 55,212-2 for 24hrs
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2-OS)
Loading amount 25 µg
Specification U2-OS
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Gracias por contactarnos.

La concentración de este anticuerpo es dependiente del lote. He conseguido localizar el único pedido que tenemos de la Univ. de XXXXX de este producto para comprobar el lote enviado, que si no me equivo...

Read More

For the detection of total ERK1+2 we recommend ab17942, a Rabbit polyclonal directed against amino acids 317-339 of Human ERK1+ERK2. This region is not subject to phosphorylation. ab17942 is covered by our Abpromise guarantee to work in ICC/IF, IHC-Fr,...

Read More

Thank you for contacting us. The concentration of antibody ab17942 in lot GR46650-5 is 0.5 mg/mL. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (cortex)
Loading amount 25 µg
Specification cortex
Gel Running Conditions Reduced Denaturing (14% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification NIH3T3 Mouse Fibroblasts
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Intestine)
Loading amount 20 µg
Specification Mouse Intestine
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. J. Davies

Verified customer

Submitted Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Embryonic stem cells-induced neurosphere)
Specification Embryonic stem cells-induced neurosphere
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"