Overview

  • Product nameAnti-ERK1 + ERK2 antibody
    See all ERK1 + ERK2 primary antibodies
  • Description
    Rabbit polyclonal to ERK1 + ERK2
  • Tested applicationsWB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    A synthetic peptide from the internal part of ERK1 + ERK2 conjugated to an immunogenic carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab76163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
  • Application notesIHC-P: 1/300 - 1/2000. Antigen retrieval is recommended.
    IHC-Fr: 1/300 - 1/2000.
    WB: 1/300 - 1/2000. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ERK 1 antibody
      • ERK 2 antibody
      • ERK antibody
      • ERK-2 antibody
      • ERK1 antibody
      • erk1/2 antibody
      • ERK2 antibody
      • ERT1 antibody
      • ERT2 antibody
      • Extracellular signal regulated kinase 1 antibody
      • Extracellular signal regulated kinase 2 antibody
      • Extracellular signal-regulated kinase 2 antibody
      • HS44KDAP antibody
      • HUMKER1A antibody
      • Insulin stimulated MAP2 kinase antibody
      • MAP kinase 1 antibody
      • MAP kinase 2 antibody
      • MAP kinase isoform p42 antibody
      • MAP kinase isoform p44 antibody
      • MAPK 1 antibody
      • MAPK 2 antibody
      • MAPK 3 antibody
      • MAPK1 antibody
      • MAPK2 antibody
      • MAPK3 antibody
      • MGC20180 antibody
      • Microtubule associated protein 2 kinase antibody
      • Mitogen activated protein kinase 1 antibody
      • Mitogen activated protein kinase 2 antibody
      • Mitogen activated protein kinase 3 antibody
      • Mitogen-activated protein kinase 1 antibody
      • Mitogen-activated protein kinase 2 antibody
      • MK01_HUMAN antibody
      • p38 antibody
      • p40 antibody
      • p41 antibody
      • p42-MAPK antibody
      • PRKM 1 antibody
      • PRKM 2 antibody
      • PRKM 3 antibody
      • PRKM1 antibody
      • PRKM2 antibody
      • PRKM3 antibody
      • Protein kinase mitogen activated 1 antibody
      see all

    References for Anti-ERK1 + ERK2 antibody (ab76163)

    ab76163 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (hippocampus)
    Loading amount 25 µg
    Specification hippocampus
    Gel Running Conditions Reduced Denaturing (14% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted May 31 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"