Overview

  • Product nameAnti-ERK1 + ERK2 antibody [ERK-7D8]
    See all ERK1 + ERK2 primary antibodies
  • Description
    Mouse monoclonal [ERK-7D8] to ERK1 + ERK2
  • Specificityab54230 reacts strongly with both ERK1 and ERK2.
  • Tested applicationsWB, IP, ELISA, EMSA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Zebrafish
    Predicted to work with: Cow, Carp, Chimpanzee, Sea Urchin, Opossum
  • Immunogen

    Synthetic peptide:

    EALAHPYLEQYYDPTDEPVAEE

    , corresponding to internal sequence amino acids 324-345, a segment near the C-terminus of Rat ERK1

  • Positive control
    • Human A431 cell lysates, Human K562 cell lysates, Canine MDCK cells lysates, Mouse brain lysate, Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab54230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
IP Use at 2-5 µg/mg of lysate.
ELISA Use a concentration of 0.1 - 1 µg/ml.
EMSA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

Anti-ERK1 + ERK2 antibody [ERK-7D8] images

  • All lanes : Anti-ERK1 + ERK2 antibody [ERK-7D8] (ab54230) at 1/1000 dilution

    Lane 1 : Human granulosa cell line whole cell lysate
    Lane 2 : Human granulosa cell line whole cell lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    IRDye® 680-conjugated goat anti-mouse IgG1 (H+L) polyclonal at 1/25000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa


    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

References for Anti-ERK1 + ERK2 antibody [ERK-7D8] (ab54230)

This product has been referenced in:
  • Chamberlain CE  et al. Menin determines K-RAS proliferative outputs in endocrine cells. J Clin Invest 124:4093-101 (2014). WB ; Mouse . Read more (PubMed: 25133424) »
  • Sun D  et al. Sox9-related signaling controls zebrafish juvenile ovary-testis transformation. Cell Death Dis 4:e930 (2013). IHC ; Zebrafish . Read more (PubMed: 24263104) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (Human granulosa cell Line)
Specification Human granulosa cell Line
Permeabilization Yes - Ethanol and Triton
Fixative Paraformaldehyde
Username

Mr. FRANCESCO ELIA MARINO

Verified customer

Submitted Jun 19 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Human Cell lysate - whole cell (Human Granulosa cell line)
Specification Human Granulosa cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Mr. FRANCESCO ELIA MARINO

Verified customer

Submitted Jun 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"