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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus ERK1 + ERK2.
(Peptide available as ab108469.)
Our Abpromise guarantee covers the use of ab94484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42, 44 kDa).Can be blocked with ERK1 + ERK2 peptide (ab108469).|
|ICC/IF||Use a concentration of 5 µg/ml.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab94484 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab94484 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"