Anti-ERK1 + ERK2 (phospho Y180) antibody (ab78238)

Overview

  • Product nameAnti-ERK1 + ERK2 (phospho Y180) antibodySee all ERK1 + ERK2 primary antibodies ...
  • Description
    Sheep polyclonal to ERK1 + ERK2 (phospho Y180)
  • SpecificityPhosphorylated ERK1 (42 kDa) and ERK2 (44kDa).
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: CTGFLTEYpVAT, corresponding to amino acids 174-183 of Human ERK1 + ERK2

  • Positive control
    • WB: lysates from serum-stimulated mouse NIH/3T3 cells, from NGF-stimulated rat PC-12 cells and EGF-stimulated human A431 carcinoma cells. IP: EGF stimulated A431 RIPA lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab78238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.1 - 2 µg/ml. Predicted molecular weight: 42 , 44 kDa.
IP Use at 8 µg/mg of lysate.

Target

  • FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ERK 1 antibody
    • ERK 2 antibody
    • ERK antibody
    • ERK-2 antibody
    • ERK1 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal regulated kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • MAPK1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • MGC20180 antibody
    • Microtubule associated protein 2 kinase antibody
    • Mitogen activated protein kinase 1 antibody
    • Mitogen activated protein kinase 2 antibody
    • Mitogen activated protein kinase 3 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42 MAPK antibody
    • p42-MAPK antibody
    • p42MAPK antibody
    • p44 ERK1 antibody
    • p44 MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 1 antibody
    • PRKM 2 antibody
    • PRKM 3 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • Protein kinase mitogen activated 1 antibody
    • Protein kinase mitogen activated 2 antibody
    • Protein kinase mitogen activated 3 antibody
    • Protein tyrosine kinase ERK 2 antibody
    see all

Anti-ERK1 + ERK2 (phospho Y180) antibody images

  • All lanes : Anti-ERK1 + ERK2 (phospho Y180) antibody (ab78238) at 2 µg/ml

    Lane 1 : Lysate from serum-stimulated 3T3 cells
    Lane 2 : Lysate from non-stimulated 3T3 cells

    Secondary
    Rabbit anti-Sheep, HRP-conjugated at 1/1000 dilution
    developed using the ECL technique

    Predicted band size : 42 , 44 kDa
    Observed band size : 42,44 kDa

References for Anti-ERK1 + ERK2 (phospho Y180) antibody (ab78238)

ab78238 has not yet been referenced specifically in any publications.

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